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Crayfish eDNA Service

Since 2015 SureScreen have been researching and developing an eDNA test for white-clawed crayfish (Austropotamobius pallipes) and after extensive testing of the primers developed by our molecular biologists in house, the service is now ready.

SureScreen are currently the only laboratory that can test for white-clawed crayfish using eDNA.

SureScreen can also test for signal crayfish (Pacifastacus leniusculus), crayfish plague (Aphanomyces astaci) in the same test, which can be extremely useful depending on your project. Other species of crayfish such as narrow clawed crayfish (Astacus leptodactylus), red swamp crayfish (Procambarus clarkii) and rusty crayfish (Orconectes rusticus) can also be tested for on request.

 

Sampling

Our kits here include everything you need to take a sample. SureScreen have managed to develop a disposable single use kit using a syringe and DNA filter which prevents the need for hiring heavy pumps and vastly reduces the risk of contamination between sites.

Crayfish eDNA Season: 1st April to 30th October

Although SureScreen have been able to pick up crayfish eDNA throughout the whole year, the concentration and therefore the probability of picking up crayfish DNA in a body of water reduces when the species are in a torpid state or are less active from November to April.

Included in the kits is a sample collection form with a summary method of how to take an effective sample as well as a detailed method to ensure best results in various situations.

 

Analysis

After collection, samples need to be frozen as soon as possible. Samples must also be kept cold during transit, this is why there is an ice pack included in the kit.

The analysis is carried out in a similar manner to the Natural England approved method of analysis eDNA for GCN, save that the DNA is extracted from the filter and we can analysis for one, two or three of the targets below.

Target species:

  1. White clawed crayfish          Austropotamobius pallipes.
  2. Signal crayfish                       Pacifastacus leniusculus.
  3. Crayfish plague                      Aphanomyces astaci.

 

Costs

Kit

£25.00 including free next day delivery

 

Analysis

One Target:     £140.00

Two Targets:   £170.00

Three Targets: £200.00

Turnaround times are up to 10 working days from receiving the sample at the laboratory. Costs exc vat.

 

FAQs

Click here to see our most frequently asked questions.

 

More information

For more information see our crayfish presentation here.

 

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